# Diabetes / Diabetologia Reproducibility Checklist
_generated 2026-05-02 UTC by IsletPerifusionAnalyzer_

Item | Requirement | Reported value
---- | ----------- | --------------
Sample source | species, sex, donor ID / strain, age, BMI | primary_mouse_islet; INS-1_832/13; iPSC-SC-beta_D21; primary_human_islet; primary_mouse_islet
IEQ / cell number / protein | report which normalization basis was used | IEQ basis = 100.0
Culture conditions | media, glucose, FBS, days post-isolation | [fill in]
Perifusion system | vendor (BioRep / Brandel / in-house), chamber volume, tubing length | in-house
Flow rate | mL/min and stability check (CV < 5%) | 0.1 mL/min
Dead volume + transit time | uL and computed lag (min) | 100.0 uL → 1.00 min
Stimulation protocol | glucose steps (mM, min), secretagogues (uM, min) | 2.8/16.7 mM glucose 10/30 min + 30 mM KCl 10 min
Reference KCl peak | 30 mM, intra-batch CV (target <= 15%) | 30 mM, CV 11.49%
Assay | ELISA / Luminex / HTRF; LLOD; ULOQ; intra-/inter-assay CV | [fill in]
Standard curve | linear range, R^2, fit method | [fill in]
Degradation correction | storage hours, decay rate applied | [fill in]
Baseline subtraction | pre-stimulus window, mean +- 2SD | [fill in]
Data exclusions | criteria for failed channels | [fill in]
Statistical test | n, replicates, test, multiplicity correction | [fill in]
Code + raw data | DOI / repo for raw CSV + analysis scripts | [fill in]

## Auto-detected pass/fail
- KCl intra-batch CV: **11.49** (threshold <= 15%)
- KCl normalization gate: **PASS**
- Channels analyzed: 25
- Files parsed: 5

## Disclaimer
본 도구는 연구·참고용이며 임상 의사결정에 직접 사용 금지.