# Methods (auto-draft)

Islet perifusion was performed using a in-house perifusion system at 0.10 mL/min with a chamber dead volume of 100 µL (transit-time lag = 1.00 min). Following a 10-min basal period at 2.8 mM glucose, samples were stimulated sequentially with 16.7 mM glucose (10–40 min), 30 mM KCl (40–50 min), and returned to 2.8 mM glucose (50–60 min). 5 file(s) and 25 channel(s) were analyzed. Traces were time-shifted to correct for transit lag, drift-corrected for storage-time-dependent peptide degradation, and normalized to IEQ where reported. Inter-batch normalization used the 30 mM KCl peak with intra-batch CV = 11.5% (target ≤ 15%). 1st-phase response was defined as 10–20 min, 2nd-phase as 20–40 min. AUC was computed by trapezoidal integration over 0–10, 10–30, and 30–60 min windows.

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